Synthesis of photoreactive phosphatidic acid analogues displaying activatory properties on cyclic AMP-phosphodiesterases. Photoaffinity labeling of an isoform of …

M Picq, Y Huang, M Lagarde, A Doutheau…

Index: Picq, Madeleine; Huang, Yan; Lagarde, Michel; Doutheau, Alain; Nemoz, Georges Journal of Medicinal Chemistry, 2002 , vol. 45, # 8 p. 1678 - 1685

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Citation Number: 12

Abstract

We have previously shown that phosphatidic acid (PA) is a specific activator of some isoforms of type 4 cyclic nucleotide phosphodiesterases (PDE 4) and that accumulation of endogenous PA can, in this way, influence the cAMP signaling pathway in different cell types. Enzyme activation depends on direct binding of the effector to specific sites carried by the enzyme. To identify the binding domain, photoactivatable phosphatidic acid ...