Photochemical coenzyme regeneration in an enzymatically active optical material

…, PL Chang, AJ Tobin, JI Zink, B Dunn

Index: Rickus, Jenna L.; Chang, Pauline L.; Tobin, Allan J.; Zink, Jeffrey I.; Dunn, Bruce Journal of Physical Chemistry B, 2004 , vol. 108, # 26 p. 9325 - 9332

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Citation Number: 17

Abstract

The photoinduced electron transfer between immobilized thionine and the dinucleotide enzyme cofactors NADH and NADPH in a SiO2 sol-gel matrix is reported. The electron- transfer quenching of thionine luminescence is used to monitor the rate of NADPH oxidation. Using Stern-Volmer quenching curves, the quenching rates in the silica matrix are 1 to 2 orders of magnitude smaller than those in solution. The rate constants for oxidation of ...