Molecules

Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins

Y Ma, H Biava, R Contestabile, N Budisa, ML Di Salvo

Index: Ma, Ying; Biava, Hernan; Contestabile, Roberto; Budisa, Nediljko; Di Salvo, Martino Luigi Molecules, 2014 , vol. 19, # 1 p. 1004 - 1022

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Citation Number: 16

Abstract

Abstract: In this paper, we present a novel,“single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non- canonical amino acids from simple precursors, coupled with expanded genetic code. In particular, we engineered the intracellular biosynthesis of l-azidohomoalanine from O-acetyl- l-homoserine and NaN 3, and achieved its direct incorporation into recombinant target ...