4, 15-Diacetylnivalenol (DANIV) was produced in liquid culture and purified by fractionation over silica followed by preparative liquid chromatography. Hydrolysis gave nivalenol (NIV), a trichothecene mycotoxin found as a common contaminant in grain grown in the North Island of New Zealand. Reaction of NIV with succinic anhydride in the presence of n-butylboronic acid and (dimethy1amino) pyridine produced a mixture of three mono-and two ...