Biochemistry

Spectroscopic, steady-state kinetic, and mechanistic characterization of the radical SAM enzyme QueE, which catalyzes a complex cyclization reaction in the …

RM McCarty, C Krebs, V Bandarian

Index: McCarty, Reid M.; Krebs, Carsten; Bandarian, Vahe Biochemistry, 2013 , vol. 52, # 1 p. 188 - 198

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Citation Number: 25

Abstract

7-Carboxy-7-deazaguanine (CDG) synthase (QueE) catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5, 6, 7, 8-tetrahydropterin (CPH4) to CDG in the third step of the biosynthetic pathway to all 7-deazapurines. Here we present a detailed characterization of QueE from Bacillus subtilis to delineate the mechanism of conversion of CPH4 to CDG. QueE is a member of the radical S-adenosyl-l-methionine (SAM) ...