Organic letters

In vitro precursor-directed synthesis of polyketide analogues with coenzyme a regeneration for the development of antiangiogenic agents

MI Kim, SJ Kwon, JS Dordick

Index: Kim, Moon I.I.; Kwon, Seok Joon; Dordick, Jonathan S. Organic Letters, 2009 , vol. 11, # 17 p. 3806 - 3809

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Citation Number: 20

Abstract

Polyketide analogues are produced via in vitro reconstruction of a precursor-directed polyketide biosynthetic pathway. Malonyl-CoA synthetase (MCS) was used in conjunction with chalcone synthase (CHS), thereby allowing efficient use of synthetic starter molecules and malonate as extender. Coenzyme-A was recycled up to 50 times. The use of a simple immobilization procedure resulted in up to a 30-fold higher yield of pyrone CHS products ...