A quantitative evaluation of methods for coupling asparagine

S Mojsov, AR Mitchell, RB Merrifield

Index: Mojsov, Svetlana; Mitchell, Alexander R.; Merrifield, R. B. Journal of Organic Chemistry, 1980 , vol. 45, # 4 p. 555 - 560

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Citation Number: 71

Abstract

A quantitative procedure was developed to evaluate methods for coupling asparagine. Boc- asparagine was coupled to glycyloxymethylphenoxymethyl-copoly (st~ ene~ iviny1benzene) resin under a variety of conditions, and the product, Asn-Gly, and any byproducts were cleaved from the resin with 50% trifluoroacetic acid in methylene chloride. The mixture was then separated on a sulfonated ion exchange column and quantitated by the ninhydrin ...