New colorimetric substrates for the assay of glycosidases

KH Aamlid, G Lee, BV Smith, AC Richardson…

Index: Aamlid, Kai H.; Lee, Grantham; Smith, Brian V.; Richardson, Anthony C.; Price, Robert G. Carbohydrate Research, 1990 , vol. 205, # 1 p. C5 - C9

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Citation Number: 13

Abstract

The preferred method of detecting and assaying enzymes is to incubate with a substance which is as close to the natural substrate as possible, but which will liberate a molecule that can be quantified on the basis of its colour, fluorescence, or chemiluminescence. The last two properties are particularly sensitive, but their measurement requires specialised equipment. The most widely used substrates for the assay of glycosides are the 4-and ...