Molecular BioSystems

A fluorescent amino acid probe to monitor efficiency of peptide conjugation to glass surfaces for high density microarrays

Y Zhao, MC Pirrung, J Liao

Index: Zhao, Yongfeng; Pirrung, Michael C.; Liao, Jiayu Molecular BioSystems, 2012 , vol. 8, # 3 p. 879 - 887

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Citation Number: 3

Abstract

Using a fluorescent NBD amino acid, new protease substrates were developed that are attractive because of the excellent chemical stability and long wavelength of excitation (480 nm) of the NBD fluorophore. The fluorescent peptides are synthesized by Fmoc solid-phase peptide synthesis. An example peptide was efficiently immobilized onto a microarray surface using click chemistry, and its proteolysis was monitored by fluorescence imaging. ...