Journal of Chromatography A 2004-03-12

On-capillary complexation of metal ions with 4-(2-thiazolylazo)resorcinol in capillary electrophoresis.

Min Wang, Jin-Ming Lin, Feng Qu, Xiaoquan Shan, Zuliang Chen

Index: J. Chromatogr. A. 1029(1-2) , 249-54, (2004)

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Abstract

Co(II), Zn(II), Ni(II) and Fe(I) were successfully separated by capillary electrophoresis using pre-capillary and on-capillary complexation with 4-(2-thiazolylazo)resorcinol. The influences of some crucial parameters, including both pre- and on-capillary complexation procedure, were investigated. For on-capillary complexation, the complexing reaction was carried out inside the capillary by mixing the zones of ligand and sample during the electrophoretic migration. Compared with pre-capillary complexation, the method provided 30-fold reduction in detection limits for Co(II), 50-fold reduction for Zn(II), and 100-fold reduction for Ni(II) and Fe(II). It was used for the analysis of a pharmaceutical and tap water sample.