Clinical Biochemistry 2014-10-01

Deproteination of whole blood for LC–MS/MS using paramagnetic micro-particles

Dorien Van den Bossche, Nele Peersman, Koen Desmet, Pieter Vermeersch, Steven Pauwels

Index: Clin. Biochem. 47(15) , 112-5, (2014)

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Abstract

Objectives Liquid chromatography tandem mass spectrometry has become increasingly popular in clinical laboratories over the last decade due to the inherent sensitivity and specificity of the technology. Nevertheless, full automation and hence application in routine laboratories is still hampered by laborious and difficult-to-automate sample pre-treatment protocols. Functionalized paramagnetic micro-particles could simplify sample pre-treatment and ease automation. We evaluated the applicability of a pre-commercial, straightforward paramagnetic micro-particle based kit for the lysis and deproteination of whole blood for the LC–MS/MS analysis of everolimus and compared the performance to our routine protein precipitation method.