Analytical Biochemistry 2014-07-01

Colorimetric detection of melamine in milk by citrate-stabilized gold nanoparticles.

Naveen Kumar, Raman Seth, Harish Kumar

Index: Anal. Biochem. 456 , 43-9, (2014)

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Abstract

Here, we report a simple and sensitive colorimetric method for detection of melamine in milk using gold nanoparticles (AuNPs). AuNPs of 21-nm size were synthesized by the citrate reduction method. The method is based on the principle that the melamine causes the aggregation of AuNPs and, hence, the wine red color of AuNPs changes to blue or purple. This change in color can be visualized with the naked eye or an ultraviolet-visible (UV-Vis) spectrometer. Under optimized conditions, AuNPs are highly specific for melamine and can detect melamine down to a concentration of 0.05 mg L(-1).Copyright © 2014 Elsevier Inc. All rights reserved.