Macromolecular Bioscience 2015-05-01

Three-Layered Biodegradable Micelles Prepared by Two-Step Self-Assembly of PLA-PEI-PLA and PLA-PEG-PLA Triblock Copolymers as Efficient Gene Delivery System.

Daniel G Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Olivia M Merkel, Tomoko Fujiwara

Index: Macromol. Biosci. 15 , 698-711, (2015)

Full Text: HTML

Abstract

"Three-layered micelles" (3LM) composed of two triblock copolymers, poly(L-lactide)-b-polyethyleneimine-b-poly(L-lactide) (PLLA-PEI-PLLA) and poly(L-lactide)-b-poly(ethylene glycol)-b-poly(L-lactide) (PLLA-PEG-PLLA) are designed to combine electrostatic interaction and solvent-induced condensation of DNA. The low molecular weight PLLA-PEI-PLLA is synthesized by a facile amine-protection/deprotection approach and employed as a gene vector, compacting DNA as a polyplex core in the organo-micelles. The individual organo-micelle is further encapsulated within a PLLA-PEG-PLLA amphiphilic micelle leading to an aqueous stable colloidal dispersion. The resulting spherical 3LM possess a hydrodynamic diameter of ca. 200 nm and zeta potential close to neutral and display excellent stability to competing polyanions such as dextran sulfate in neutral pH (7.4). Such high stability is attributed to the complete shielding of the PEI/DNA polyplex core with an impermeable hydrophobic intermediate layer. However, greater than 90% of the encapsulated DNA are released within 30 min when exposed to slightly acidic pH (4.5). Based on our findings, a new class of non-viral delivery system for nucleic acids with superb stability and stealth properties is identified. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Related Compounds

Structure Name/CAS No. Articles
Chloroform Structure Chloroform
CAS:67-66-3
Acetonitrile Structure Acetonitrile
CAS:75-05-8
Methanol Structure Methanol
CAS:67-56-1
Di-tert-butyl dicarbonate Structure Di-tert-butyl dicarbonate
CAS:24424-99-5
trifluoroacetic acid Structure trifluoroacetic acid
CAS:76-05-1
Chlorobenzene Structure Chlorobenzene
CAS:108-90-7
Thiazolyl Blue Structure Thiazolyl Blue
CAS:298-93-1
Tin dioctoate Structure Tin dioctoate
CAS:301-10-0