Toxicology 2013-01-07

Analysis of microRNA and gene expression profiling in triazole fungicide-treated HepG2 cell line.

Yu Ri An, Seung Jun Kim, Moon-Ju Oh, Hyun-Mi Kim, Il-Seob Shim, Pil-Je Kim, Kyunghee Choi, Seung Yong Hwang

Index: Toxicology 303 , 94-8, (2013)

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Abstract

MicroRNA (miRNA) plays an important role in various diseases and in cellular and molecular responses to toxicants. In the present study, we investigated differential expression of miRNAs in response to three triazole fungicides (myclobutanil, propiconazole, and triadimefon). The human hepatoma cell line (HepG2) was treated with the above triazoles for 3 h or 48 h. miRNA-based microarray experiments were carried out using the Agilent human miRNA v13 array. At early exposure (3h), six miRNAs were differentially expressed and at late exposure (48 h), three miRNAs were significantly expressed. Overall, this study provides an array of potential biomarkers for the above triazole fungicides. Furthermore, these miRNAs induced by triazoles could be the foundation for the development of a miRNA-based toxic biomarker library that can predict environmental toxicity.Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.