Nonacid cleavable detergents applied to MALDI mass spectrometry profiling of whole cells

…, MJ Hangauer, NA Porter, RM Caprioli

Index: Norris, Jeremy L.; Hangauer, Matthew J.; Porter, Ned A.; Caprioli, Richard M. Journal of Mass Spectrometry, 2005 , vol. 40, # 10 p. 1319 - 1326

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Citation Number: 12

Abstract

Abstract Although cleavable detergents were first synthesized a number of years ago, they have only recently been successfully applied to problems involving biological molecules. Recent reports have demonstrated that these compounds are useful for applications involving both 2D PAGE and mass spectrometry. However, most cleavable surfactants have utilized acid-labile functional groups to affect cleavage. In applications where extreme pH ...