China Journal of Chinese Materia Medica 2012-12-01

[Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa].

Jian-Ping Xue, Tao Xue, Lan Guo, Yan-Fang Zhu, He-Dong Lu, Ai-Min Zhang, Wei Sheng

Index: Zhongguo Zhong Yao Za Zhi 37(24) , 3812-4, (2012)

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Abstract

To study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa, in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R. glutinosa.Leaves from test-tube seedlings of 85-5 R. glutinosa were used as explants. After rooting induction, they were transferred to medium with orthogonal design for inducing test-tube tuberous roots of R. glutinosa.NAA played a significant role in induction of test-tube tuberous roots of R. glutinosa, followed by sucrose and 6-BA. With leaves from test-tube seedlings as the explants, the optimal medium for inducing test-tube tuberous roots of R. glutinosa was MS + BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 7%. The study provides an efficient induction system for studies on artificial seeds and secondary metabolism with test-tube tuberous roots of R. glutinosa.