Proteomics 2009-05-01

Mass spectrometry-compatible silver staining of histones resolved on acetic acid-urea-Triton PAGE.

Khare Satyajeet Pramod, Khade Bharat, Gupta Sanjay

Index: Proteomics 9(9) , 2589-92, (2009)

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Abstract

Acetic acid-Urea-Triton (AUT) PAGE is commonly used method to separate histone variants and their post-translationally modified forms. Coomassie staining is the preferred method for protein visualization; however, its sensitivity is less than that of silver staining. Though silver staining of histones in AUT-PAGE has been reported, the method is time-consuming, dependent on prior staining by Amido black and has not been reported suitable for mass spectrometry. Here, we propose 'SDS-Silver' method for rapid, sensitive and mass spectrometry-compatible staining of histones resolved on AUT-PAGE.