Analytical Biochemistry 2012-01-01

L-amino acid oxidase-induced apoptosis in filamentous Botrytis cinerea.

Chi-Hua Cheng, Chia-Ann Yang, Shu-Ying Liu, Chaur-Tsuen Lo, Kou-Cheng Peng, Chi-Hua Cheng, Chia-Ann Yang, Shu-Ying Liu, Chaur-Tsuen Lo, Kou-Cheng Peng

Index: Anal. Biochem. 420(1) , 93-5, (2012)

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Abstract

As opposed to single-cell yeast and mammalian cell lines, apoptosis has not been greatly investigated in filamentous fungi because antibodies to the relevant fungal apoptosis-related proteins are not available commercially and because multicellular organisms cannot be studied using flow cytometry. Here we demonstrate how antibodies from a nonfungal source could be used to investigate this pathway. We show that apoptosis in the filamentous fungus Botrytis cinerea is triggered by the mitochondria-mediated caspase pathway, with release of the apoptotic factors cytochrome c, caspase 3, and caspase 9, on treatment with Trichoderma harzianum-derived L-amino acid oxidase.Copyright © 2011 Elsevier Inc. All rights reserved.