Journal of Pharmaceutical and Biomedical Analysis 2013-03-25

Improved capillary electrophoresis method for the analysis of carbohydrate-deficient transferrin in human serum, avoiding interference by complement C3.

Yukihiro Kuroda, Ryohei Hamaguchi, Kenji Moriyama, Toshiko Tanimoto, Jun Haginaka

Index: J. Pharm. Biomed. Anal. 76 , 81-6, (2013)

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Abstract

Capillary electrophoresis (CE) methods for transferrin analysis are widely used in clinical laboratories, but complement C3 peaks often overlap with carbohydrate-deficient transferrin peaks. In this study, we discovered that the electrophoretic mobility of the complement C3 peak could be controlled by adding carboxymethylcellulose (CMC) and dextran sulfate (DS) to the background electrolyte solution, probably because of adsorption of the polyanions to the proteins. The improved capillary electrophoresis method was developed using spermine, CMC, and DS as the background electrolyte solution additives. The carbohydrate-deficient transferrin concentrations determined using this system were in good agreement with those determined by HPLC, with acceptable reproducibility. These results suggest that this method has the potential to be developed into a new clinical test.Copyright © 2012 Elsevier B.V. All rights reserved.