Bioanalysis 2015-01-01

Quantification of remifentanil and propofol in human plasma: a LC-MS/MS assay validated according to the EMA guideline.

Jean Claude Alvarez, Emuri Abe, Isabelle Etting, Morgan Le Guen, Philippe Devillier, Stanislas Grassin-Delyle

Index: Bioanalysis 7 , 1675-84, (2015)

Full Text: HTML

Abstract

Remifentanil and propofol are often used in combination for general anesthesia. We developed a method using LC-MS for their simultaneous measurement in human plasma. Methodology & results: After addition of remifentanil-(13)C6 and propofol-d18 (IS), 500 µl of plasma were extracted with ethylacetate/hexane. Analysis conditions included gradient elution (water/acetonitrile), electrospray ionization and detection with a triple quadripole mass spectrometer. Remifentanil and propofol were ionized in the positive and negative mode, respectively. The method was validated according to the European Medicines Agency guideline for the validation of bioanalytical methods, then successfully applied to clinical samples from three patients who had undergone liver transplantation.This method is suitable for the simultaneous quantification of remifentanil and propofol in clinical studies.

Related Compounds

Structure Name/CAS No. Articles
N-hexane Structure N-hexane
CAS:110-54-3
Acetonitrile Structure Acetonitrile
CAS:75-05-8
Methanol Structure Methanol
CAS:67-56-1
Propofol Structure Propofol
CAS:2078-54-8
Formic Acid Structure Formic Acid
CAS:64-18-6
Citric Acid Structure Citric Acid
CAS:77-92-9
SodiuM bicarbonate Structure SodiuM bicarbonate
CAS:144-55-8
ethyl acetate Structure ethyl acetate
CAS:141-78-6
Propionic acid Structure Propionic acid
CAS:79-09-4
2-Ethylaniline Structure 2-Ethylaniline
CAS:578-54-1