Chemical Research in Toxicology 2012-10-15

IL-8 release from human neutrophils cultured with pro-haptenic chemical sensitizers.

Eva Kiorpelidou, Brian Foster, John Farrell, Monday O Ogese, Lee Faulkner, Chris E Goldring, B Kevin Park, Dean J Naisbitt

Index: Chem. Res. Toxicol. 25(10) , 2054-6, (2012)

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Abstract

Cytokine release from dendritic cells in vitro is a useful marker to discriminate between sensitizing and irritant haptenic chemicals. Unfortunately, pro-haptens, which gain reactivity following metabolic/auto activation, yield negative results. To overcome this, we exposed human neutrophils and THP-1 cells to haptens/pro-haptens and measured IL-8 release. Haptenic compounds stimulated IL-8 release in neutrophils and THP-1 cells. In contrast, the pro-haptens eugenol, isoeugenol, and 2-aminophenol stimulated high levels of IL-8 release from neutrophils alone. Neutrophil cytokine release was reduced when glutathione was added. Cyp1A1/1B1/3A4 were not detectable in THP-1 cells or neutrophils; however, neutrophils expressed high levels of myeloperoxidase.