Journal of Biochemical and Biophysical Methods 1992-12-01

HPLC analysis of hexosamine phosphates in biological samples.

S Ambrosio, J R De Oliveira, R Bartrons

Index: J. Biochem. Biophys. Methods 25(4) , 237-44, (1992)

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Abstract

Galactosamine is quickly metabolized to galactosamine 1-phosphate in rats treated with this compound. An HPLC method to quantify hexosamine phosphates in biological samples is described, modified from the o-phthaldialdehyde amino acid analysis procedure. o-Phthaldialdehyde derivatives of hexosamines and hexosamine-phosphates can be eluted from a reverse-phase column at different retention times, with a total analysis time of 30 min and without overlapping with free amino acids at physiological concentrations. The standard curves are linear between 1 and 40 nmol. This simple method is more selective and sensitive than previous enzymatic analyses of hexosamine phosphorylation.