American Journal of Veterinary Research 1985-12-01

Rapid extraction, radioiodination, and in vivo catabolism of 125I-labeled fibrinogen in the horse.

C P Coyne, W J Hornof, A B Kelly, T R O'Brien, S J DeNardo

Index: Am. J. Vet. Res. 46(12) , 2578-81, (1985)

Full Text: HTML

Abstract

Two methods were analyzed for the rapid extraction of equine fibrinogen from fresh plasma, using ammonium sulfate-sodium phosphate buffer. Fibrinogen from each of these 2 methods was then radiolabeled with 125I (half-life = 60.2 days, gamma = 35 keV), using monochloroiodine reagent. Mean protein-bound activity was 98.5% and mean clottable radioactivity was 94.1%. Radiolabeled fibrinogen administered IV to 15 horses had an overall mean (+/- SD) plasma half-life of 4.95 +/- 0.44 days.