FEBS Letters 2014-01-03

N-Terminal signal sequence is required for cellular trafficking and hyaluronan-depolymerization of KIAA1199.

Hiroyuki Yoshida, Aya Nagaoka, Sachiko Nakamura, Megumi Tobiishi, Yoshinori Sugiyama, Shintaro Inoue

Index: FEBS Lett. 588(1) , 111-6, (2014)

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Abstract

Recently, we disclosed that KIAA1199-mediated hyaluronan (HA) depolymerization requires an acidic cellular microenvironment (e.g. clathrin-coated vesicles or early endosomes), but no information about the structural basis underlying the cellular targeting and functional modification of KIAA1199 was available. Here, we show that the cleavage of N-terminal 30 amino acids occurs in functionally matured KIAA1199, and the deletion of the N-terminal portion results in altered intracellular trafficking of the molecule and loss of cellular HA depolymerization. These results suggest that the N-terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199-mediated HA depolymerization. Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.