International Journal of Systematic and Evolutionary Microbiology 2014-05-01

Salegentibacter chungangensis sp. nov., isolated from a sea sand and emended description of the genus Salegentibacter.

Chatuphon Siamphan, Wonyong Kim

Index: Int. J. Syst. Evol. Microbiol. 64(Pt 5) , 1514-9, (2014)

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Abstract

A Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterial strain, designated CAU 1289T, was isolated from a marine sand and its taxonomic position was investigated using a polyphasic approach. It grew optimally at pH 6.5 and 30 °C and in the presence of 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1289T belonged to the genus Salegentibacter, exhibiting sequence similarity values of 93.6-96.3% to members of this genus, and was related most closely to Salegentibacter mishustinae KMM 6049T (96.3% similarity). Strain CAU 1289T contained MK-6 as the predominant menaquinone. The major fatty acid was iso-C15:0. The cell-wall peptidoglycan of strain CAU 1289T contained meso-diaminopimelic acid. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, an unidentified phospholipid, an unidentified aminolipid, an unidentified aminophospholipid, an unidentified glycolipid and ten unidentified lipids. The DNA G+C content was 38.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic inference, strain CAU 1289T is considered to represent a novel species of the genus Salegentibacter, for which the name Salegentibacter chungangensis sp. nov. is proposed. The type strain is CAU 1289T (KCTC 24000T=CCUG 64793T). An emended description of the genus Salegentibacter is also proposed.