7-Deoxyadriamycinone 0.90 0, 70 0.35 0. 40 ethanol-acetic acid-water (179: 4: 5: 12 by volume); IV, ethyl acetate-benzene-petroleum ether (bp 40-70”)(70: 25: 5 by volume). When systems I and II were used, plates were buffered at pH 7 with phosphate buffer. The estimation of labeled compounds involved scraping of chromatogram in sections of the same length,. extracting each section with 0.5 ml of methanol in a counting vial, and ...
