Biochemical and Biophysical Research Communications 1991-06-14

ESR measurement of radical clearance in lung of whole mouse.

K Takeshita, H Utsumi, A Hamada

Index: Biochem. Biophys. Res. Commun. 177(2) , 874-80, (1991)

Full Text: HTML

Abstract

Clearance of the nitroxide radicals, hydroxy-TEMPO and carboxy-PROXYL, in whole-mouse lung was directly measured by in vivo ESR. After injecting a nitroxide radical, distribution of the nitroxide radical all over the lung was confirmed by ESR imaging. The ESR signal of hydroxy-TEMPO was reduced in the lung and the clearance obeyed first-order kinetics, whereas the signal of carboxy-PROXYL remained constant. Comparison of the clearance rates of live and dead mice indicated the presence of 2 different clearance systems in the lung: loss of its paramagnetism in the lung, and transfer from alveolar to the blood circulation system.