Journal of Chromatography A 2008-01-01

Use of cyclohexanedimethanol as a nonflammable organic solvent for industrial scale reversed phase chromatography

Alan K. Hunter, Sydney D. Hoeltzli, Gary V. Johnson, Mark E. Gustafson, Sa V. Ho, Alan K. Hunter, Sydney D. Hoeltzli, Gary V. Johnson, Mark E. Gustafson, Sa V. Ho

Index: J. Chromatogr. A. 1202(1) , 107-10, (2008)

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Abstract

This work demonstrates that cyclohexanedimethanol is an effective nonflammable organic solvent for the pilot scale reversed phase chromatography of recombinant apolipoprotein A-I Milano. Cyclohexanedimethanol has low viscosity in water, enabling the use of conventional low pressure process scale chromatography columns and hardware. Results from pilot scale manufacturing using a 30 cm diameter CG71M packed column indicate that a 5.7 log reduction in E. coli host cell protein with >80% yield can be obtained for the purification of apolipoprotein A-I Milano.