Clinica Chimica Acta 1985-06-30

Fluorometric measurement of glycosylated albumin in human serum.

Y Hayashi, M Makino

Index: Clin. Chim. Acta 149(1) , 13-9, (1985)

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Abstract

A simple and rapid fluorometric assay of glycosylated albumin was developed using dansylated phenyl boronic acid (N-(5-dimethyl amino-1-naphthalene sulfonyl)-3-aminobenzene boronic acid). The emission spectrum of the reagent changes upon binding to cis-diols present on glycosylated albumin in serum, allowing quantitation of glycosylated albumin. Results from the new procedure correlated well with those from commercially prepared affinity chromatography (r = 0.85).