Bioorganic & Medicinal Chemistry Letters 1999-01-18

Design and synthesis of reagents for phage display screening of dehalogenases.

R J Pieters, M Fennema, R M Kellogg, D B Janssen

Index: Bioorg. Med. Chem. Lett. 9(2) , 161-6, (1999)

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Abstract

Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane dehalogenase enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.