FEBS Letters 2014-05-02

Biochemical and structural characterisation of a haloalkane dehalogenase from a marineRhodobacteraceae

Halina R. Novak, Christopher Sayer, Michail N. Isupov, Dorothee Gotz, Andrew Mearns Spragg, Jennifer A. Littlechild

Index: FEBS Lett. 588(9) , 1616-22, (2014)

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Abstract

• Isolation of a marine Rhodobacteraceae sp. from a tube worm. • Identification of a putative haloalkane dehalogenase enzyme. • Characterisation and crystal structure of the native haloalkane dehalogenase enzyme and product bound complex. • Relationship between the substrate specificity and the size and charge distribution within the active site cavity.