Analytical Biochemistry 2007-05-15

Capillary electrophoretic determination of selenocyanate and selenium and tellurium oxyanions in bacterial cultures.

Bala Krishna Pathem, Gonzalo A Pradenas, Miguel E Castro, Claudio C Vásquez, Thomas G Chasteen

Index: Anal. Biochem. 364(2) , 138-44, (2007)

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Abstract

A simple capillary zone electrophoretic method for the determination of biospherically important oxyanions of selenium (Se) and tellurium and another Se-containing anion, selenocyanate, has been developed. The method uses direct UV absorption detection. Time course experiments with time slices as short as 6 min are possible. This method's detection limits and linear range compare well with other methods involving samples containing complex biological matrices. The metalloid-containing anions examined were selenocyanate, selenite, selenate, tellurite, and tellurate. We applied this method to live cultures of two different bacteria in two different growth media in time course experiments following the changes in metalloid-containing anion concentrations. The results show that this method is a useful means of following the biological processing of these analytes in bacterial cultures.