Protein Expression and Purification 2012-06-01

Enhanced soluble expression of recombinantFlavobacterium heparinumheparinase I inEscherichia coliby fusing it with various soluble partners

Jing Huang, Lin Cao, Wanhua Guo, Ruoxi Yuan, Zhijun Jia, Kehe Huang, Jing Huang, Lin Cao, Wanhua Guo, Ruoxi Yuan, Zhijun Jia, Kehe Huang

Index: Protein Expr. Purif. 83(2) , 169-76, (2012)

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Abstract

Highlights ► IF2 and SUMO can significantly improve the soluble expression of HepA. ► The fusion proteins can be conveniently purified by a single step of IMAC. ► Fusion proteins of HepA retain their biological activity. ► Native HepA can be obtained by efficient enterokinase digestion.