Food Hygiene and Safety Science/Shokuhin Eiseigaku Zasshi 2001-02-01

[Determination of neohesperidin dihydrochalcone in foods].

M Nakazato, C Kobayashi, Y Yamajima, M Kawano, K Yasuda

Index: Shokuhin Eiseigaku Zasshi 42(1) , 40-4, (2001)

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Abstract

An HPLC method was developed for the determination of neohesperidin dihydrochalcone (NHDC) in foods. A solid sample was extracted with methanol. The extract was evaporated and the residue was dissolved in methanol-water (2:8) mixture. In the case of fluid sample, the sample solution was prepared by dilution with methanol-water (2:8) mixture. The sample solution was cleaned up on a C18 cartridge. The cartridge was washed with water and methanol-water (3:7) mixture, and NHDC was eluted with methanol-water (7:3) mixture. NHDC in the eluate was separated on an ODS column and determined with a UV detector (282 nm). The identity of NHDC in foods was confirmed by means of HPLC with a photodiode-array detector. The recoveries of NHDC added to various kinds of foods were 75.2-134%. The determination limit of NHDC was 1 microgram/g.