Food and Chemical Toxicology 2009-10-01

Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes.

Chang-Suk Kong, Jung-Ae Kim, Se-Kwon Kim

Index: Food Chem. Toxicol. 47(10) , 2401-6, (2009)

Full Text: HTML

Abstract

In this study, we investigated the effect of sulfated glucosamine (SGlc) on adipogenesis of 3T3-L1 adipocytes during differentiation of preadipocytes into adipocytes by measuring lipid accumulation and adipogenesis related factors. Treatment with SGlc reduced the triglyceride content in Oil-Red O staining and enhanced glycerol secretion in adipocytes in a dose-dependent manner. In addition, SGlc induced the down-regulation of adipogenesis related factors and adipocyte specific gene promoters. Moreover, treatment of 3T3-L1 adipocytes with SGlc activated the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) alpha and beta along with their substrate, acetyl-CoA carboxylase (ACC). These results suggest that inhibitory effect of SGlc on adipocyte differentiation might be mediated through the up-regulation of AMPK pathway.