Journal of Microbiological Methods 2003-03-01

Removal of PCR inhibitors from soil DNA by chemical flocculation.

Michael D Braid, Laura M Daniels, Christopher L Kitts

Index: J. Microbiol. Methods 52(3) , 389-93, (2003)

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Abstract

Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH(4)(SO(4))(2) during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.