Chemical Communications 2002-07-07

A screening system for enantioselective enzymes based on differential cell growth.

Manfred T Reetz, Carsten J Rüggeberg

Index: Chem. Commun. (Camb.) (13) , 1428-9, (2002)

Full Text: HTML

Abstract

The esterase-catalyzed enantioselective hydrolysis of the fluoroacetate of pantolactone leads to fluoroacetic acid, a toxic compound which inhibits the growth of esterase-producing yeast; this forms the basis of an ee-assay.