Sudebno-meditsinskaia ekspertiza 2010-01-01

[Detection of bancol for the purpose of forensic chemical expertise].

V K Shormanov, Iu N Baranov, E P Duritsyn, S V Maslov, E I Pronichenko, S V Ganiev

Index: Sud. Med. Ekspert. 53(6) , 39-41, (2010)

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Abstract

Optimal conditions for the extraction of bancol from the biological material with toluene are described. The possibility of its purification and separation from co-extracted compounds on a silicagel L column, 40/100 mcm is illustrated. Identification and quantitative determination of bancol isolated from the cadaverous liver were performed by the electron spectrophotometry technique, thin-layer chromatography, and high performance liquid chromatography using normal-phase sorbents. A method of bancol detection was adapted for the purpose of forensic medical expertise and applied for the postmortem examination of the cadaverous tissues.