Applied and Environmental Microbiology 2008-09-01

Purification, molecular cloning, and enzymatic properties of a family 12 endoglucanase (EG-II) from fomitopsis palustris: role of EG-II in larch holocellulose hydrolysis.

Tomoko Shimokawa, Hajime Shibuya, Masanobu Nojiri, Shigeki Yoshida, Mitsuro Ishihara

Index: Appl. Environ. Microbiol. 74(18) , 5857-61, (2008)

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Abstract

A family 12 endoglucanase with a molecular mass of 23,926 Da (EG-II) from the brown-rot basidiomycete Fomitopsis palustris was purified and characterized. One of the roles of EG-II in wood degradation is thought to be to loosen the polysaccharide network in cell walls by disentangling hemicelluloses that are associated with cellulose.