Pharmazie 1996-09-01

Solid-phase extraction and HPLC analysis of kebuzone and its metabolites in blood.

J Klimes, J Sochor, J Sedlacek

Index: Pharmazie 51(9) , 651-3, (1996)

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Abstract

A HPLC method for quantification of kebuzone and its metabolites in whole blood was developed. The compounds and the internal standard were isolated from blood by solid-phase extraction on a C-18 cartridge. A blood sample was to be hemolyzed before extraction. HPLC was performed on a C-18 column with the mobile phase composed of methanol/water acidified to pH 2.7 and UV absorbance detection at 247 nm. This method has been successfully applied to a pharmacokinetic study of kebuzone and its metabolites in rabbits.