Insect Biochemistry and Molecular Biology 1995-12-01

Developmental studies on Drosophila melanogaster glutathione S-transferase and its induction by oxadiazolone.

A A Hunaiti, A M Elbetieha, M A Obeidat, W M Owais

Index: Insect Biochem. Mol. Biol. 25(10) , 1115-9, (1995)

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Abstract

Glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene was detected in various developmental stages of Drosophila melanogaster. The specific activity of the enzyme was 110, 35, 25 and 15 nmol/min/mg protein in crude extracts prepared from eggs, larvae, pupae and adult stages respectively. The enzymes from larval, pupal and adult stages were purified and compared. Incorporation of the widely used herbicide oxadiazolone at concentrations of 375 and 563 part/million into the culture media caused 4- and 2.5-fold increase in the enzyme activity in pupal and adult stages respectively.