Protein & Peptide Letters 2002-10-01

The use of DODT as a non-malodorous scavenger in Fmoc-based peptide synthesis.

A Teixeira, W E Benckhuijsen, P E de Koning, A R P M Valentijn, J W Drijfhout

Index: Protein Pept. Lett. 9(5) , 379-85, (2002)

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Abstract

We have synthesized a random group of peptides and performed cleavages using various cleavage cocktails including 3,6-dioxa-1,8-octanedithiol (DODT). Purity of the peptides was compared to that obtained with standard protocols for cleavage using RP-HPLC and Maldi-Tof mass spectrometry. We show that stinking thiols can be replaced by the almost odourless (DODT) without negatively affecting the purity of the end product.