Bulletin of Experimental Biology and Medicine 2011-12-01

Phytochemical study of the north caucasian Rosa spinossima L. fruit.

O O Novikov, D I Pisarev, E T Zhilyakova, M U Novikova, E V Bondarenko, D A Fadeeva, M D Bezmenova

Index: Bull. Exp. Biol. Med. 152(2) , 216-8, (2011)

Full Text: HTML

Abstract

Qualitative composition and content of anthocyanins in the Rosa spinossima L. fruit were studied. The anthocyanin sum was isolated by talc adsorption with subsequent desorption. The presence of anthocyanins in the resultant fraction was confirmed by thin layer chromatography. Three spots corresponding to anthocyanins were found. Anthocyanins were identified by mass spectrometry. Ion peaks corresponding to glycoside cyanidin and delphinidin were found. Position of sugar in the glycoside structure was determined by UV spectrophotometry with aluminum chloride. The content of anthocyanins was measured by direct UV spectrophotometry by the anthocyanins absorption. Anthocyanin content in raw material in conversion to cyanidin-3-glucoside was 1.46±0.03%.