Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 1993-02-01

Isolation of Antho-RFamide related peptides from the eyestalks of blue crab.

A Yasuda, Y Naya, K Nakanishi

Index: Comp. Biochem. Physiol.,. B. 104(2) , 235-40, (1993)

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Abstract

1. Two carboxyl-terminally amidated peptides (CP1 and CP2) were isolated from the blue crab (Callinectes sapidus) eyestalks by a method of carboxyl-terminal analysis. 2. The peptides were sequenced as pGlu-Gly-Arg-Phe-amide (CP1) and pGlu-Leu-Gly-Arg-Phe-amide (CP2). 3. Each carboxyl-terminus of the peptides was precisely determined by amino acid analysis utilizing phenylisothiocyanate derivatives. 4. CP1 was identical to the sea anemone neuropeptide, Antho-RFamide.