Bioorganic & Medicinal Chemistry Letters 2012-07-01

Polymerase incorporation of pyrene-nucleoside triphosphates.

Marcel Hollenstein, Filip Wojciechowski, Christian J Leumann

Index: Bioorg. Med. Chem. Lett. 22(13) , 4428-30, (2012)

Full Text: HTML

Abstract

Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.Copyright © 2012 Elsevier Ltd. All rights reserved.