O Périn-Roussel, N Barat, M J Plessis, F Zajdela
Index: Cancer Lett. 61(3) , 207-13, (1992)
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The formation of DNA adducts was investigated in mouse fibroblasts from two different tissues--embryos and adult lung--after incubation with dibenzo[a,e]fluoranthene (DBF) or its major proximate metabolites. The nuclease P1 modification of the 32P-postlabeling method was adapted for detection of DBF-DNA adducts. Quantitative and qualitative differences were observed in the metabolic activation mediated by the two cell types. DBF-DNA adducts generated three major spots reproducibly, and more than ten spots of medium or weak importance. The highest level of DNA binding occurred via the DBF-bay region vicinal dihydrodiol epoxide but with significant differences in the quantitative distribution of adducts. Striking qualitative differences were observed when lung fibroblasts were incubated with the DBF-pseudo bay region dihydrodiol (DBF-12,13-DHD). The spots representing adducts induced in embryo fibroblasts by DBF-3OH-12,13-DHD, a further metabolite of DBF-12,13-DHD, were totally absent from chromatograms of lung cells. These results show that both embryo and lung fibroblasts can activate DBF but that different cytochrome P-450 forms and substrate affinities are involved. The finding that different activation systems may be present in subcategories of the same tissue, may provide a partial explanation for the wide variations in sensitivity to carcinogens among species, organs and tissues.
Structure | Name/CAS No. | Molecular Formula | Articles |
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Indeno[1,2,3-gh]tetraphene
CAS:5385-75-1 |
C24H14 |
Dibenzo[a,e]fluoranthene.
1983-12-01 [IARC Monogr. Eval. Carcinog. Risk Chem. Hum. 32 , 321-5, (1983)] |
Metabolic activation of dibenzo(a,e)fluoranthene, a nonalter...
1980-05-01 [Cancer Res. 40(5) , 1742-9, (1980)] |
Apparent stimulation of dibenzo[a,e]fluoranthene in vitro me...
1981-10-01 [Chem. Biol. Interact. 37(1-2) , 109-22, (1981)] |
DNA-protein crosslinks induced by exposure of cultured mouse...
1984-03-01 [Carcinogenesis 5(3) , 379-83, (1984)] |
Multi-step metabolism of the carcinogen dibenzo[a,e]fluorant...
1983-01-01 [Carcinogenesis 4(7) , 827-35, (1983)] |
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