Electrophoresis 1994-06-01

Improved separation of diastereomeric derivatives of enantiomers by a physical network of linear polyvinylpyrrolidone applied as pseudophase in capillary zone electrophoresis.

W Schützner, G Caponecchi, S Fanali, A Rizzi, E Kenndler

Index: Electrophoresis 15(6) , 769-73, (1994)

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Abstract

Diastereomeric analytes were separated using capillary zone electrophoresis with polyvinylpyrrolidone as polymeric additive to the buffer solution. As test substances derivatives of D- and L- alpha-amino and alpha-hydroxy acids formed by reaction with (+)-O,O'-diacetyl- and (+)-O,O'-dibenzoyl-L-tartaric anhydride, respectively, were used. The physical network formed by the linear polymer is supposed to act as a kind of pseudophase. It was found that the network affects the mobility of diastereomeric compounds to a different extent, enhancing the selectivity of the system. In nearly all cases of aromatic acids the diastereomer carrying the D-amino acid was more strongly retained than the L-isomer, as opposed to the situation with aliphatic acids.