Journal of Microbiological Methods 2004-09-01

Binding interactions of Escherichia coli with globotetraosylceramide (globoside) using a surface plasmon resonance biosensor.

Mohammad R Pourshafie, Britt-Inger Marklund, Sten Ohlson

Index: J. Microbiol. Methods 58(3) , 313-20, (2004)

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Abstract

Surface plasmon resonance with an alkane L1 chip was used to investigate the binding of uropathogenic Escherichia coli, carrying adhesion receptors, to globotetraosylceramide (globoside; GbO4). The immobilization of globoside was reproducible and resulted in a stable globoside layer on the L1 chip. The data indicated that the globoside-immobilized L1 chip could be used for studying interactions with live or chemically fixed E. coli. The results indicated that the dissociation time was significantly reduced in glutaraldehyde-fixed E. coli as compared to living cells. Overall, the report demonstrates the significance of the L1 chip in terms of sensitivity, specificity, handling, and speed when studying globoside/E. coli interactions. This model may assist in screening for compounds that can inhibit the binding of uropathogenic E. coli to glycolipid ligands on target cells.