Journal of Chromatography B 2005-09-25

Detection of mercaptopyridines and mercaptopyrimidines in planar chromatography with iodine-azide reaction as a detection system.

Robert Zakrzewski, Witold Ciesielski

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 824(1-2) , 222-8, (2005)

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Abstract

Reaction between iodine and azide ion induced by mercaptopyridines and mercaptopyrimidines was utilized as a detection system in TLC and HPTLC. The developed plates were sprayed with a freshly prepared mixtures of sodium azide and starch solution adjusted to pH 5.5, and exposed to iodine vapour. The spots became visible as white spots on violet-grey background. The iodine-azide detection system has been proved to be the most favourable and enabled to detect quantities per spot in the range of 1-20 pmol (HPTLC) and 1-60 pmol (TLC). The iodine-azide tests were compared with other visualizing techniques commonly used in planar chromatography (iodine vapour and UV254). The developed method was applied to detection of thiopental in biological samples.