Canadian Journal of Microbiology/Revue Canadienne de Microbiologie 2006-09-01

Characterization of the glyoxalase I gene from the vascular wilt fungus Verticillium dahliae.

A Klimes, M J Neumann, S J Grant, K F Dobinson

Index: Can. J. Microbiol. 52 , 816-822, (2006)

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Abstract

A glyoxalase I gene homologue (VdGLO1) was identified in the vascular wilt fungus Verticillium dahliae by sequence tag analysis of genes expressed during resting structure development. The results of the current study show that the gene encodes a putative 345 amino acid protein with high similarity to glyoxalase I, which produces S-D-lactoylglutathione from the toxic metabolic by-product methylglyoxal (MG). Disruption of the V. dahliae gene by Agrobacterium tumefaciens-mediated transformation resulted in enhanced sensitivity to MG. Mycelial growth of disruption mutants was severely reduced in the presence of 5 mmol/L MG. In contrast, spore production in liquid medium was abolished at 1 mmol/L MG, although not at physiologically relevant concentrations of